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          GPM Cyclone, advanced search form, running on BEETLE single thread

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  1. 1. spectra
    common, mzXML, mzData, DTA, PKL or MGF only
      

    taxon
    Select one or more.
    Eukaryotes Prokaryotes Viruses
    1. Include reversed sequences:
    2. all 15N amino acids
    | none | mixed | only |
    with peptide log(e) < with protein log(e) <
    gpmdb
    1. Add to gpmDB: yes restricted no
    2. Archive MS/MS informationyes no
    3. Anonymous contribution: yes no

    4.   more ...
  2. 2. measurement errors
    1. Fragment mass error:  
    2. Parent mass error: + -
    3. Isotope error: yes no
    4. Fragment type: monoisotopic average
  3. 3. signal processing
    1. Remove redundant: yes no, angle: (0-90)
    2. Maximum parent charge:
    3. Spectrum synthesis: yes no
    4. Noise suppression: yes no
    5. Minimum parent M+H:
    6. Minimum fragment m/z:
    7. Total peaks:
    8. Minimum peaks:
    9. Fragment types: a b c x y z
  4. 4. protein modifications
    1. Complete modifications (unimod)
      Set 1

      specify your own
      Set 2

      more sets ...
    2. Potential modifications (unimod)
      specify your own
    3. Potential motif:
    4. Protein N-terminus:  Da
    5. Protein C-terminus:  Da
    6. Use sequence annotations yes no
  5. 5. refinement specification
    1. Refine model: yes no
    2. Point mutations: yes no
    3. Use sequence annotations yes no
    4. Semi-style cleavage: yes no
    5. Potential modifications (unimod):
      round 1 round 2

       mods:
      motifs:

       mods:
      motifs:
      round 3 round 4

       mods:
      motifs:

       mods:
      motifs:
    6. Use these modifications throughout: yes no
    7. Unantipated cleaves ([X]|[X]): yes no
    8. Potential N-terminus modifications:
    9. Potential C-terminus modifications:
    10. Valid expectation: <
  6. 6. protein cleavage specification
    1. Cleavage site:

      specify your own
    2. Semi-style cleavage: yes no
    3. Missed cleavage sites allowed:
    4. Cleavage C-terminal change:  Da
    5. Cleavage N-terminal change:  Da